Antagonistic Potential of Lactobacillus Spp against Enteropathogenic Bacteria; Purification and Characterization of their Bacteriocins

In the present study, Lactobacillus (160) isolates were isolated from curd sample. The isolates were aimed to analyze the antibacterial potential against Escherichia coli, Vibrio cholerae sub sp., ogawa, V. cholerae sub sp., inaba, Klebsiella sp., Proteus sp. and Shigella dysenteriae. All the isolates were inhibiting the tested Enteropathogenic bacteria except S. dysenteriae. Lactobacillus isolates produced highest inhibition zone (30 to 37 mm) against V. cholerae sub sp., inaba and Klebsiella sp., of the 160 isolates only ten Lactobacillus isolates (L1L10) were used for the production of bacteriocins, purified by ammonium sulphate precipitation and ion exchange (DEAE cellulose) chromatography. Maximum bacteriocin activity has been observed with Lf3 against V. cholerae ssp Inaba at 30°C, pH 6.0, 1.5 to 2.0% Na Cl/18 h in addition to L8, L9 and L10 (MW 100 to 106 KDa) and Lf3 was found to be the most prominent potential isolate.